Bioquote Limited

NEW!! ThioStar® Thiol Detection Reagent  50 µg Catalogue Number L002-50UG  125.00 UK Pounds 110µg Catalogue Number L002-110UG 240.00 UK Pounds

Most Sensitive Fluorescent Thiol Reagent Available From non-Fluorescent to Brightly Fluorescent in Seconds Use in microplates or cuvettes

The ThioStar™ molecule is designed to quantitatively measure thiol groups generated or present in biological samples. ThioStar covalently binds to any free thiol groups to yield a highly fluorescent product. After mixing the sample with ThioStar and incubating at room temperature for 30 minutes, the fluorescent product is read at 510 nm in a fluorescent plate reader or fluorometer with excitation at 390 nm.

Free thiols in biological systems have important roles. Oxidatively modified thiol groups of cysteine residues are known to modulate the activity of a growing number of proteins. One of the most pressing problems with this approach is to accurately determine the extent of modification of specific amino acids, such as cysteine residues, in a complex protein sample.

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