GRS Viral DNA/RNA Purification Kit

The GRS Viral DNA/RNA Purification Kit provides an efficient and fast method for the purification of high quality viral DNA and RNA from cell-free media (e.g. from serum, body fluids, and the supernatant of viral infected cell cultures). Eluted purified Nucleic Acid is suitable for all common applications, including PCR, real-time PCR, RT-PCR, One-step qRT-PCR, and DNA Sequencing. This kit is recommended for parallel purification of viral DNA (including CMV and HBV) and viral RNA (including HIV, HTLV, HCV, and SARS-CoV-2). The detection limit depends on the type of virus and on the sensitivity of individual PCR or RT-PCR protocols.

Principle
The GRS Viral DNA/RNA Purification Kit is optimized to eliminate the need of an internal control or carrier RNA. Lysis of DNA/RNA viruses using the Viral Lysis Buffer is fast and efficient. The buffer system is optimized to allow selective binding of nucleic acids to the glass fiber matrix of the spin-column (1). Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified DNA/RNA is subsequent eluted with RNase-Free Water. The entire procedure can be completed within 30 minutes without the use of phenol extraction.

NOTE: Due to COVID-19 outbreak and consequent high demand of this product, lead time may be longer than usual. Please contact us via sales@bioquote.com

NOTE :  This product is temporarily not supplied with individually packed columns but rather with columns in bags of 50 units that have been treated with GAMA rays.

Sample : 200 µl of cell-free medium (serum,  body fluids, supernatant of viral infected cells), swab samples, sputum, and nasopharyngeal/oropharyngeal/tracheal washes/aspirates
Expected Yield : viral DNA and RNA (101-109 copies)
Format : spin column  (certified DNase/RNase-free)
Operation Time : within 30 minutes
Elution Volume : 50 μl

Catalogue Number : GK12.0100 (100 Preps) For Pricing Please Call.