A4FB-Phosphoramidite
The S-4FB (N-succinimidyl-4-formylbenzamide) heterobifunctional crosslinker is fundamental to Solulink’s proprietary linker technology. The crosslinker is used for direct incorporation of 4FB (4-formylbenzamide) moieties on oligonucleotides during solid phase synthesis. The S-4FB analog reacts with primary amines on proteins (amino group of lysine), amino-modified oligonucleotides or surfaces, introducing a 4FB (4-formylbenzamide) linker that forms stable covalent conjugates with biomolecules possessing HyNic (6-hydrazinonicotinamide) incorporated linkers. 4FB groups incorporated on biomolecules and surfaces are extremely stable and have been known to maintain their reactivity for >18 months.
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