Cofilin 2 Antibody
Background
Cofilin 2 Antibody. This antibody is expected to recognise both reported isoforms (NP_068733 and NP_619579)
Additional Names
CFL2; cofilin 2 (muscle) ; HGNC:1875 ; cofilin 2
DescriptionLeft: Western blot analysis of cofilin 2 in Human Muscle lysate (35μg protein in RIPA buffer) using cofilin 2 antibody (0.003μg/ml).
Source
Cofilin 2 antibody was raised against a synthetic peptide near the C-terminus of cofilin 2.
Purification
Cofilin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Cofilin 2 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 18kDa band observed in Human Duodenum, Heart and Muscle lysates (calculated MW of 18.7kDa according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03µg/ml
Tested Application
E, WB
Buffer
Cofilin 2 antibody is supplied as 0.1mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H,M,R,D,P
Protein Accession Number
NP_068733.1; NP_619579.1
Data Sheet Version
08-01W
References
- Thirion C, Stucka R, Mendel B, Gruhler A, Jaksch M, Nowak KJ, Binz N, Laing NG, Lochmuller H. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem. 2001 Jun;268(12):3473-82
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