Human PPARα Reporter Assay System, 3 x 32 assays in 96-well format

Product Overview:
This nuclear receptor assay system uses specific non-human cells modified to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or PPARα. Indigo’s reporter cells include the luciferase reporter gene functionally linked to a PPARα-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PPARα activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human PPARα. PPARα reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. Indigo Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to PPARα reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. IB0011

Size : 1 Kit        

Shipping : dry ice Stability : Store at -80 degrees; shelf life 180 days maximum after production Application(s):

human

Specificity:

• Peroxisome Proliferator-Activated Receptor Alpha (PPARα; NR1C1)

Custom Code: 3822000002 UNSPSC code: 41116

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