Immuno Histo Chemistry – Hsp90alpha and Hsp90beta 160 Slides Kit
Picture shows Anti-Hsp90α. The human colon tissue stained (brown) mostly in inflammatory cells. Shown at a 1:10,000 dilution of SKC-243 using the ABC method.
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now known to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(3). When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (7).
Certified in Mouse and Human, other species nottested. For details on the species reactivities of the antibodies used,please see individual data sheets corresponding to the catalog numbersgiven on the kit components tab.
1. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572-52577.
2. Pearl H., et al. (2001) Adv Protein Chem 59: 157-186.
3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
4. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133.
5. Pratt W., Toft D. (1997) Endocr Rev 18: 306-360.
6. Pratt W.B.. (1998) Proc Soc Exptl Biol Med 217: 420-434.
7. Whitesell L., et al. (1994) Proc. Natl. Acad. Sci. USA 91:8324-8328.
Kit Components : (For Full Kit Cat No. SKT-290RB)
SKC-243 Anti-Hsp90alpha Antibody (Special format of SMC-137, a mouse monoclonal) (50ul)
SKC-244 Anti-Hsp90beta Antibody (Special format of SMC-107, a mouse monoclonal) (50ul)
SKC-903 Amplifying IHC Wash Buffer (20x) (500ml)
SKC-905 Epitope Unmasking Solution (10x) (250ml)
SKC-902 Amplifying Antibody Dilution Buffer (500ml)
SKC-900 Antibody Amplifier 1(12wells)
SKC-901 Antibody Amplifier Eclipse for IF 1(12wells)
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