Immuno Histo Chmistry – CamKIIphospho 160 Slides Kit

Picture Shows Anti-Phospho-CaMKII visualized in colon cancer tissue, shown at a 1:5,000 dilution of SKC-221 using the ABC method.

Description :

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Data :

Certified in Mouse and Human, other species not tested. For details on the species reactivities of the antibodies used,please see individual data sheets corresponding to the catalog numbersgiven on the kit components tab.

References :

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008-36013.

2. Barria A. et al. (1997) Science 276: 2042-2045.

3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.

4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.

5. Nghiem P., Saati S.M., Martens C.L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.

6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.

7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.

8. Means A.R. (2000) Mol. Endocrinol. 14: 4-12.

9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500-2510.

10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.

11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.

12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

13. Shifman J.M., Choi M.H., Mihalas S, Mayo S.L., Kennedy M.B. 2006 Proc. Natl. Acad. Sci. U.S.A. 103: 13968-13973

Kit Components : (For Full Kit Cat No. SKT-221RB)

SKC-221 Anti-Phospho-CaMKII Antibody (Special format of SMC-125, a mouse monoclonal) (100ul)

SKC-903 Amplifying IHC Wash Buffer (20x) (500ml)

SKC-905 Epitope Unmasking Solution (10x) (250ml)

SKC-902 Amplifying Antibody Dilution Buffer (500ml)

SKC-900 Antibody Amplifier 1(12wells)

SKC-901 Antibody Amplifier Eclipse for IF 1(12wells)

Download datasheet