Lumigen ECL Ultra (TMA-6)

Lumigen ECL Ultra (TMA-6) Description :

Lumigen ECL Ultra (TMA-6) is an ultra-sensitive chemiluminescent substrate formulated for the detection of horseradish peroxidase (HRP) conjugates on membranes. Designed for high-performance Western blotting applications, Lumigen ECL Ultra delivers exceptional signal intensity and sustained light output, enabling precise visualization of low-abundance protein targets.

Upon reaction with HRP-labeled antibodies, the substrate produces a rapid and highly luminous chemiluminescent signal. Its strong signal generation supports short exposure times while maintaining clarity and band definition. The sustained emission profile allows flexible imaging windows, supporting both digital imaging systems and film-based detection workflows.

Lumigen ECL Ultra is optimized for high-contrast detection across a broad dynamic range. It enables reliable visualization from nanogram to picogram protein levels, making it well suited for applications requiring enhanced sensitivity without sacrificing signal stability. The reagent supports crisp, well-defined band resolution and low background when used in properly optimized blotting systems.

Supplied as a two-component system (Solution A and Solution B) mixed in a 1:1 ratio to form the working substrate, Lumigen ECL Ultra reflects Lumigen’s decades of expertise in chemiluminescent chemistry and reagent formulation. It is trusted by research laboratories and assay developers seeking consistent, high-intensity detection performance.

Invented, developed, and manufactured by Lumigen, Lumigen ECL Ultra continues the company’s legacy of delivering reliable, high-sensitivity visible-light detection solutions for life science research.

Lumigen ECL Ultra (TMA-6) Specifications :
Product Name : Lumigen® ECL Ultra (TMA-6)
Product Type : Chemiluminescent Reagent
Catalog Number : TMA-100, TMA-1000
Contents : Solution A + Solution B; Mix 1:1 for working solution
Target Enzyme : Horseradish Peroxidase (HRP)
Primary Application : Ultra-sensitive detection of HRP-conjugated antibodies in Western blotting
Detection Method : Enhanced chemiluminescence (ECL)
Signal Characteristics : High intensity; Short exposure times (15–30 sec); Imaging possible for several hours
Assay Format : Western blot membrane-based detection
Working Solution Preparation : Mix Solutions A and B 1:1; Incubate membrane 2–3 minutes before imaging
Readout Equipment : X-ray film or CCD imager
Storage Conditions : 2–8°C; Protect from direct light; Do not freeze
Handling Requirements : Use in subdued light; Equilibrate to room temperature before mixing; Avoid membrane drying
Shelf Life : Stable when stored at 2–8°C protected from light (per manufacturer guidelines)
Container Requirements (If Repackaged) : New opaque HDPE or PP plastic containers only
Repackaging Guidance : Repackaging discouraged to prevent contamination and elevated background

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