Pancreas Lysate

Background : Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Tissue Type : Pancreas

Diagnosis : Normal

Source : Human pancreas tissue

Tested Application : WB

Buffer : Pancreas lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.

Storage : Lysate is supplied at a concentration of 2 mg/ml. Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

Species Reactivity : N/A

Species : Human

Short Description : total protein normal pancreas – human

This product belongs to the following categories:

• Lysates
• Human Lysates
• Human Normal Tissue Lysates
• Human Total Protein Lysates
• Human Adult Lysates
• Human Pancreas Tissue Lysates
• Tissue Lysates
• Normal Tissue Lysates
• Total Protein Lysates
• Adult Lysates
• Pancreas Tissue Lysates

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• Pancreas Nuclear Lysate
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Datasheet 0802-W

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