Phi29 DNA Polymerase
Cat. No. PHI250, PHI1250
Phi29 DNA polymerase (10 units/㎕, 0.1 ㎍/㎕)
NanoHelix Phi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3´→5´ exonuclease activity that enables proofreading capability.
25 mM NaH2PO4 (pH 7.0),150 mM NaCl,125mM Imidazole, 2.5 mM 2-Mercaptoethanol, 50% Glycerol
10 units/㎕, 0.1 ㎍/㎕
By heating at 65℃ for 15 minutes
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