Phi29 DNA Polymerase

NanoHelix Co. Ltd

Cat. No. PHI250, PHI1250

Phi29 DNA polymerase (10 units/㎕, 0.1 ㎍/㎕)

Description

NanoHelix Phi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. Phi29 DNA polymerase contains a 3´→5´ exonuclease activity that enables proofreading capability.

Storage buffer

25 mM NaH2PO4 (pH 7.0),150 mM NaCl,125mM Imidazole, 2.5 mM 2-Mercaptoethanol, 50% Glycerol

Concentration

10 units/㎕, 0.1 ㎍/㎕

Incubation

30℃

Inactivation

By heating at 65℃ for 15 minutes

Storage

-20℃

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£95.00 / €133.00 PHI250

£275.00 / €385.00 PHI1250

All prices shown are exclusive of VAT