ProteoSpin On-Column Proteolytic Digestion Kit
The ProteoSpin™ On-Column Proteolytic Digestion Kit provides a fast and simple procedure for the generation of purified peptides. The kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin, and the subsequent purification of the resulting peptides. Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Both the proteins and nonactive enzyme are bound to the column, while salts and other non-specifically bound materials are washed away. The trypsin is then activated, and peptides are generated and purified on the column.
Each column in the ProteoSpin™ On-Column Protelytic Digestion Kit is able to process, digest and purify samples containing up to 15 µg of protein. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications.
ProteoSpin™ On-Column Proteolytic Digestion Kit Contents :
Each kit provides essential components for use with the provided protocols.
- Column Equilibration and Wash Buffer
- Column pH Binding Buffer
- Column Activation Buffer
- Micro Spin Columns
- Elution Tubes
- Product Insert
Customer-supplied Reagents and Equipment
- Benchtop microcentrifuge
- pH indicator paper
- 37 C incubator
- Trypsin (sequencing grade)
All prices shown are exclusive of VAT