Rac1,2,3 G-LISA Activation Assay, Colorimetric

Cytoskeleton Inc

Product Uses Include :

  • Rac signaling pathway studies
  • Rac activation assays with primary cells
  • Studies of Rac activators and inactivators
  • Rac activation assays with limited material
  • High throughput screens for Rac activation

Introduction :
The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. For a more detailed introduction on G-LISA™ assays and a listing of other available G-LISA™ kits, see our main G_LISA page. The Rac1,2,3 G-LISA™ Activation Assay measures the entire level of GTP-loaded Rac1,2 and 3 protein in cell lysates, this is in contrast to Cat.# BK126 which measures only Rac1 activation levels. The level of activation is measured with absorbance at 490nm. For a kit to measure RhoA activation please check webpage BK124.

The Rac G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples. See this section on our G-LISA™ information resource page.

Citations :

BK125 was introduced in November 2006 so there are no examples of this kit being cited in publications as of the last update of this web page. However, examples of publications that have cited the use of Cat.# BK124, a similar format of G-LISA, are described below:

Warren JC, Rutkowski A. and L. Cassimeris. 2006. Infection with replication-deficient adenovirus induces changes in the dynamic instability of host cell microtubules. Mol. Biol. Cell, 17, p3557-3568.

Woods A and Beier F. 2006. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem. May 12; 281(19):13134-40.

Shipping Conditions : Ambient Temperature

Storage Conditions : 4C

Kit contents :
The kit contains sufficient reagents to perform 96 Rac activation assays. Since the Rac-GTP affinity wells are supplied as strips and the strips can be broken into smaller pieces, each kit can be used for anywhere from one to multiple assays. The following components are included in the kit:

  1. 96 Rac-GTP affinity wells (divisible into 12 strips of 8 wells each)
  2. Lysis buffer
  3. Binding buffer
  4. Antigen presenting buffer
  5. Wash buffer
  6. Antibody dilution buffer
  7. Anti-Rac antibody
  8. HRP-labeled secondary antibody
  9. Positive control Rac protein
  10. Protease inhibitor cocktail
  11. Absorbance detection reagents
  12. Precision Red™ Advanced protein assay reagent (Cat. # ADV02)
  13. Manual with detailed protocols and extensive troubleshooting guide
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96 Assay Kit

£863.10 / €1,208.34 BK125

All prices shown are exclusive of VAT