RhoA G-LISA Activation Assay, Luminescence

Cytoskeleton Inc

Product Uses Include :

  • Rho signaling pathway studies
  • Rho activation assays with primary cells
  • Studies of Rho activators and inactivators
  • Rho activation assays with limited material
  • High throughput screens for Rho activation

Introduction :
This G-LISA™ Rho activation assay measures the combined levels of GTP-loaded RhoA, RhoB and RhoC in cells. The level of activation is measured with luminometry. The G-LISA Rho activation assays are ELISA based Rho activation assays with wich you can measure Rho activity in cells in less than 3 h. For a more detailed introduction on G-LISA™ assays and a listing of other available G-LISA™ kits, see our main G-LISA page. For a kit to measure RhoA activation with absorbance detection, see Cat. # BK124. For kits to measure the combined activation of RhoA, RhoB and RhoC, see Cat. # BK123 (absorbance).

Citations:

Higashibata A, Imamizo-Sato M, Seki M, Yamazaki T. and Ishioka N. 2006. Influence of simulated microgravity on the activation of small GTPase Rho involved in cytoskeletal formation – molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor. BMC Biochemistry, 7, (19), p1-9.

Woods A. and Beier F. 2006. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J. Biol. Chem. 281, (19), p.13134-13140.

Shipping Conditions : Ambient Temperature

Storage Conditions : 4C

Kit contents :
The kit contains sufficient reagents to perform 96 Rho activation assays. Since the Rho-GTP affinity wells are supplied as strips and the strips can be broken into smaller pieces, each kit can be used for anywhere from one to multiple assays. The following components are included in the kit:

  1. 96 Rho-GTP affinity wells
  2. Lysis buffer
  3. Binding buffer
  4. Antigen presenting buffer
  5. Wash buffer
  6. Antibody dilution buffer
  7. Anti-RhoA antibody
  8. HRP-labeled secondary antibody
  9. Positive control RhoA protein
  10. Protease inhibitor cocktail
  11. Luminescence detection reagents
  12. Precision Red™ Advanced protein assay reagent (Cat. # ADV02)
  13. Manual with detailed protocols and extensive troubleshooting guide
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96 Assay Kit

£863.10 / €1,208.34 BK121

All prices shown are exclusive of VAT