S-4FB Crosslinker (DMF Soluble)

The S-4FB (N-succinimidyl-4-formylbenzamide) heterobifunctional crosslinker is fundamental to Solulink’s proprietary linker technology. S-4FB reacts with primary amines on proteins (amino group of lysine) or amino-modified oligonucleotides or surfaces, introducing a 4FB (4-formylbenzamide) linker that forms stable covalent conjugates with biomolecules possessing HyNic (6-hydrazinonicotinamide) incorporated linkers. 4FB groups incorporated on biomolecules and surfaces are extremely stable and have been known to maintain their reactivity for >18 months.

Solulink’s proprietary linker technology is based on the use of two complementary heterobifunctional linkers:  

• S-HyNic crosslinker is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
• S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
• HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

Solulink Advantages

• Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
• Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
• Stability – 10 times more stable than any other conjugation linker
• Specificity – Two-linker method avoids homoconjugate formation
• Efficient – >80% efficient linker-biomolecule conjugations
• Robust – The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0

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