S-HyNic Crosslinker (DMF Soluble)

Solulink

The S-HyNic (succinimidyl-6-hydrazino-nicotinamide) heterobifunctional crosslinker is fundamental to Solulink’s proprietary linker technology. S-HyNic reacts with primary amines on proteins (amino group of lysine) or amino-modified oligonucleotides or surfaces, introducing a HyNic (6-hydrazinonicotinamide) linker that forms stable covalent conjugates with biomolecules possessing 4FB (4-formylbenzamide) incorporated linkers.

Solulink’s proprietary linker technology is based on the use of two complementary heterobifunctional linkers:  

  • S-HyNic crosslinker is conjugated to biomolecule 1 through primary amines on proteins, oligos, peptides, or surfaces.
  • S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines on proteins, oligos, peptides, carbohydrates, or surfaces.
  • HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 to form a conjugate.

The result is two biomolecules conjugated through a UV-traceable and stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm. Thus any two proteins, regardless of molecular weight, can be efficiently conjugated.

Solulink Advantages :

  • Catalyzed Conjugation – Faster kinetics for greater efficiency and yields
  • Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
  • Stability – 10 times more stable than any other conjugation linker
  • Specificity – Two-linker method avoids homoconjugate formation
  • Efficient – >80% efficient linker-biomolecule conjugations
  • Robust – The conjugate bond is stable to 92˚C and to pH values ranging from pH 2.0–10.0
  • <

     

    <

     

    <

     

    <

    Download datasheet

Shipping Cold Gel Packs

Buy now

1x10mg

£335.00 / €469.00 S-1002-010

5x1mg

£245.00 / €343.00 S-1002-105

All prices shown are exclusive of VAT