STIM2 (IN) Antibody

Background

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

Additional Names

STIM2 (IN), Stromal interaction molecule 2

Source

STIM2 antibody was raised against a 17 amino acid peptide from near the center of human STIM2.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

STIM2 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Application

STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 – 1 μg/ml.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat.No. 4125P – STIM2 Peptide

Storage

STIM2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1288 – A20 Cell Lysate
2. Cat. No. 1306 – Human Spleen Tissue Lysate

Species Reactivity

H, M, R

Protein GI Number

41349446

Protein Accession Number

NP_065911

Short Description

(IN) an inhibitor of STIM1

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Immunology Antibodies

Related Products

• A-20 Cell Slide
  (Catalog No. 17-208).
• A-20 Lysate
  (Catalog No. 1288).
• ORAI1 Antibody
  (Catalog No. 4041).
• ORAI2 Antibody
  (Catalog No. 4111).
• ORAI3 Antibody
  (Catalog No. 4215).
• ORAI3 Antibody
  (Catalog No. 4117).
• Spleen Lysate
  (Catalog No. 1306).
• Spleen Tissue Slide (Normal)
  (Catalog No. 10-901).
• STIM1 Antibody
  (Catalog No. 4119).
• STIM2 Antibody
  (Catalog No. 4123).
• STIM2 Peptide
  (Catalog No. 4125P).

References

1. Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated ^Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
3. Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca²⁺ sensor that activates CRAC channels and migrates from the Ca²⁺ store to the plasma membrane. Nature 2005; 437:902-5.
4. Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated ^Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.

Datasheet 08-02W

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