Tubulin Protein Plus Glycerol, Bovine Brain, >99% Pure, Frozen Liquid

Cytoskeleton Inc

Product Uses Include

  • IC50 & EC50 determinations for anti-tubulin ligands.
  • Microtubule binding studies
  • HDAC6 studies
  • Microtubule activated kinesin ATPase assays

Material
Tubulin protein has been purified from bovine brain by an adaptation of the method of Shelanski et al. (1), Further purification to >99% purity was achieved by cation exchange chromatography. Tubulin is supplied at 10 mg/ml as a frozen liquid in G-PEM (General tubulin buffer (Cat. # BST01) with 1 mM GTP (Cat. # BST06)) plus 5% glycerol (Cat. # BST05).

This product is also available without glycerol in a lyophilized, fully active format (Cat. # TL238). The lyophilized format tubulin has many advantages over the frozen format. TL238 costs less and since it allows for ambient temperature shipping, shipping is also significantly less costly. In addition, the lyophilized format allows for simpler (+4°C desiccated vs -70°C) and longer storage. When TL238 is reconstituted to 10 mg.ml in G-PEM plus 5% glycerol, it is identical to T237.

>99% pure tubulin is also available in a convenient lyophilized pre-formed microtubule format (Cat. # MT001) for use in e.g. kinesin ATPase assays and microtubule binding studies.

Purity
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. Samples are >99% pure.

NOTE THIS PRODUCT REQUIRES DRY ICE SHIPPING

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20x1mg

£1,706.25 / €2,388.76 T237-E

4x250ug

£208.95 / €292.52 T237-A

1x1mg

£168.00 / €235.19 T237-B

5x1mg

£551.25 / €771.75 T237-C

10x1mg

£971.25 / €1,359.74 T237-D

All prices shown are exclusive of VAT