Family : Antibody
Formulation : 1 mg/ml in sterile PBS; BSA free, sodium azide free
Format : Purified
Clone : WLMT1-1
Host Animal : Mouse
Clonality : Monoclonal (mouse origin)
Isotype : Mouse IgG1, kappa
Species Reactivity : Human, Mouse, Rat. Other species not tested.
Application : IF, IHC-P
Application Details : Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)
Application Note : Titering of the WT1 antibody may be required for optimal performance.<p>1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen : A recombinant human protein was used as the immunogen for the WT1 antibody.
Storage : Store the WT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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