WT1 Antibody Cocktail
Family : Antibody
Formulation : 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format : Purified
Clone : WT1/857 + 6F-H2
Host Animal : Mouse
Clonality : Monoclonal (mouse origin)
Isotype : Mouse IgGs
Species Reactivity : Human, Mouse and Rat. Other species not tested.
Application : FACS, IF, IHC-P
Application Details : Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)
Application Note : Optimal dilution of the WT1 antibody cocktail should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen : Recombinant full length human protein (WT1/857) and amino acids 1-181 of human Wilms Tumor (6F-H2) were used as the immunogen for the WT1 antibody cocktail.
Storage : Store the WT1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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