Family : Antibody
Formulation : Prediluted in 1X PBS, 0.1 mg/ml BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Format : Purified
Clone : 2F3.2
Host Animal : Mouse
Clonality : Monoclonal (mouse origin)
Isotype : Mouse IgG2a, kappa
Species Reactivity : Human. Other species not tested.
Application : IHC
Application Details : Prediluted format : incubate for 30 min at RT (2)
Application Note : Optimal dilution of the ZAP-70 antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen : Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.
Storage : Store the ZAP-70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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