Family : Antibody
Formulation : 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Format : Purified
Clone : ZTP70-1
Host Animal : Mouse
Clonality : Monoclonal (mouse origin)
Isotype : Mouse IgG2a, kappa
Species Reactivity : Human. Other species not tested.
Application : IF, IHC-P
Application Details : Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)
Application Note : Titering of the ZAP-70 antibody may be required for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Immunogen : Recombinant full-length human protein was used as the immunogen for the ZAP-70 antibody.
Storage : Store the ZAP-70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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