Rat Osteocalcin EIA Kit

About the Test

The BTI Rat Osteocalcin EIA Kit is a sandwich ELISA assay.  Two osteocalcin antibodies are employed, each directed toward an end of the (C or N terminal) osteocalcin molecule.  The N-terminal monoclonal antibody is bound to the wells and when reacted with rat osteocalcin standard or sample, the C-terminal rat osteocalcin antibody completes the sandwich.  This ELISA kit shows good  correlation (r = 0.95) with our rat osteocalcin RIA.

This sandwich ELISA kit is specific for rat osteocalcin only.  Both carboxylated and decarboxylated rat osteocalcin are recognized. Rat osteocalcin can be measured directly from serum, heparinized plasma, bone extracts and serum-free cell culture supernates.

The assay can be run either overnight (0.33-20ng/ml) or in 6 hours (0.78-50ng/ml).  As little as 10 microliters of rat serum is required for duplicate determinations.  Each kit contains all the necessary reagents, an authentic rat serum control, precoated 96-well strips and a complete protocol.

BTI Rat Osteocalcin EIA Kit
Catalog No:  BT-490
(sufficient reagents and precoated 96-well strips)
Quantity : 1 kit

Specifications:
Sample Size:  100ul (diluted serum)
Assay Time:  5 hours or overnight
Sensitivity:  0.5 ng/ml
Range:  0.78-50 ng/ml or 0.33-20 ng/ml
Intraassay Variation:  4%
Interassay Variation:  7%

FOR RESEARCH USE ONLY.  NOT FOR USE IN HUMANS OR AS AN IN-VITRO DIAGNOSTIC.