PBS Buffer (10X)

Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research for washing and dissociating cells or for dissolving small molecules. Solulink’s PBS contains 0.05% sodium azide (NaN₃) and is used in the following applications:

• Washing biomolecules bound to Streptavidin Agarose High Performance resin
• Preparing antibody standards for the BCA and Bradford Assays to determine the concentration of a protein before or after modification
• Desalting proteins or antibodies prior to biotinylation or labeling
• As a blank for taking UV spec readings to determine biotin incorporation
• Washing and resuspending NanoLink and MagnaLink 4FB Magnetic Beads after antibody conjugation

Solulink’s proprietary linker technology is an innovative, catalyzed, UV-traceable, heterobifunctional linker technology that offers greater efficiency and yield in a considerably simpler method for linking any two biomolecules together e.g. proteins, oligos, or peptides regardless of molecular weight.

• S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker is conjugated to biomolecule 1 through primary amines (-NH₂) on the amino acid, lysine, or on the N-terminus.
• S-4FB (succinimidyl-4-formylbenzamide) linker is conjugated to biomolecule 2 through primary amines (-NH₂) on the amino acid, lysine, or on the N-terminus.
• HyNic-modified biomolecule 1 is incubated with 4FB-modified biomolecule 2 in a catalyzed conjugation reaction.

The result is two biomolecules conjugated through a UV-traceable, stable bond (bis-arylhydrazone) with measurable absorbance at 354 nm.

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