Human LXR Reporter Assays PANEL, 2 x 48 assays in 96-well format

Indigo Biosciences

Product Overview:
Indigo’s panel of LXR Assays uses non-human mammalian cells modified to express human estrogen receptors alpha (NR1H3) and beta (NR1H2), ligand-dependent transcription factors commonly referred to as LXRα and LXRβ. Indigo’s reporter cells include the luciferase reporter gene functionally linked to an LXR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in LXR activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against the human LXRs. LXR reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. Indigo Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to LXR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. IB0032

Size: 1 Kit        

Shipping : dry ice Stability : Store at -80 degrees; shelf life 180 days maximum after production. 

Application(s) : Human Specificity:

  • Liver X Receptors (LXRα, LXRβ)

Custom Code: 3822000002 UNSPSC code: 41116

 

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£895.00 / €1,253.00 IB00321-48P

All prices shown are exclusive of VAT