Human ERα Reporter Assay System, 1 x 384-well format assay

Product Overview:
This nuclear receptor assay system uses specific non-human cells modified to provide constitutive, high-level expression of the full-length human estrogen receptor 1 (NR3A1), a ligand-dependent transcription factor commonly referred to as ERα. Indigo’s reporter cells include the luciferase reporter gene functionally linked to an ERα-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in ERα activity. Luciferase gene expression occurs after ligand-bound ERα undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Unlike some other cell-based assay strategies, the readout from Indigo’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. ERα reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The principal application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human estrogen receptor. It is an all-inclusive assay system that includes, in addition to ERα reporter cells, two optimized media for use during cell culture and in diluting the test samples, a reference agonist, luciferase detection reagent, a cell culture-ready assay plate, and a detailed protocol. IB0040

Size : 1 Kit       

Shipping : dry ice Stability : Store at -80 degrees; shelf life 180 days maximum after production. 

Application(s) : Human Specificity:

• Estrogen Receptor Alpha (ERα; ESR1; NR3A1)

Custom Code: 3822000002 

UNSPSC code: 41116104

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