Anguizole

Short Summary : Inhibitor of HCV replication

Category : Proteases|HCV Protease

Purity : 0.98

CAS Number : 442666-98-0

Formula : C17H11ClF2N4O2S

Molecular Weight : 408.81

SMILE : C1=COC(=C1)C2=NC3=CC(=NN3C(=C2)C(F)(F)Cl)C(=O)NCC4=CC=CS4

Solubility : Soluble in DMSO

Storage : Store at -20°C

Description : Anguizole is a selective inhibitor of NS4B with IC50 value of 310 nM [1]. NS4B (nonstructural protein 4B) is a viral protein which was found in hepatitis C virus and was a key regulator in HCV replication complex formation. It is reported that NS4B plays a pivotal role in HCV replication via inducing a specific membrane rearrangement and designating membranous web. NS4B-AH2, comprising amino acid 42 to 66, is identified as a major regulator for NS4B oligomerization [2]. Anguizole is a potent NS4B inhibitor and targets the second amphipathic helix of NS4B (NS4B-AH2). When tested with HCV virus, anguizole treatment significantly disrupted NS4B-AH2-mediated biological functions of NS4B which played an important role in HCV life cycle [3]. In Huh7.5 cells transferring a plasmid expressing NS4B fused in frame with GFP, 5M anguizole treatment (48 hours) altered the distribution of NS4B MAF pattern which inhibited HCV life cycle [1].References: [1]. Bryson, P.D., et al., A small molecule inhibits HCV replication and alters NS4B’s subcellular distribution. Antiviral Res, 2010. 87(1): p. 1-8.[2]. Gouttenoire, J., et al., Aminoterminal amphipathic alpha-helix AH1 of hepatitis C virus nonstructural protein 4B possesses a dual role in RNA replication and virus production. PLoS Pathog, 2014. 10(10): p. e1004501.[3]. Choi, M., et al., A hepatitis C virus NS4B inhibitor suppresses viral genome replication by disrupting NS4B’s dimerization/multimerization as well as its interaction with NS5A. Virus Genes, 2013. 47(3): p. 395-407.

Download datasheet