IM-12

Short Summary : GSK-3? inhibitor, potent

Category : Stem Cell|GSK-3

Purity : 0.98

CAS Number : 1129669-05-1

Formula : C22H20FN3O2

Molecular Weight :

Molecular Weight : 377.41

SMILE :

Solubility : >18.9mg/mL in DMSO

Storage : Store at -20°C

Description : IM-12 is a potent inhibitor of GSK-3 with IC50 value of 53 nM [1].
Glycogen synthase kinase-3 (GSK-3) is a proline-directed serine-threonine kinase and is involved in neuronal cell development, energy metabolism and body pattern formation. Inhibition of GSK-3 can cause -catenin accumulation.
IM-12 is a potent GSK-3 inhibitor. In ReNcell VM cells, IM-12 significantly increased total -catenin by 27% and at a concentration of 3 M increased the -catenin amount most. In ReNcell VM cells, IM-12 (3 M) inhibited GSK-3 to a remaining activity of 27 5%. Also, IM-12 significantly increased the cell doubling time to 26.5 0.9 h and significantly inhibited cell proliferation. In ST14A cells, IM-12 caused an accumulation of -catenin around the nucleus. In ReNcell VM cells transfected with TOPFlash, IM-12 increased TCF-activity. In cells transfected with TOP and pCAGGS-S33Y (a vector containing -catenin), IM-12 significantly increased TCF-activity by 270%. In ReNcell VM cells, IM-12 significantly increased the level of IIItub+ cells to 3.7 0.7%, which suggested that IM-12 increased neuronal differentiation [1].
Reference:[1]. Schm le AC, Brennfhrer A, Karapetyan G, et al. Novel indolylmaleimide acts as GSK-3beta inhibitor in human neural progenitor cells. Bioorg Med Chem, 2010, 18(18): 6785-6795.

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