10x Ammonium Buffer, Mg2+ and Tween free, 6 tubes x 5 mL
Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.
Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences.
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