AEBSF.HCl

Short Summary : Serine protease inhibitor

Category : Proteases|Serine Protease

Purity : 0.9927

CAS Number : 30827-99-7

Formula : C8H10FNO2S.HCl

Molecular Weight : 239.69

SMILE : C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl

Solubility : >12mg/mL in DMSO

Storage : Desiccate at -20°C

Description : AEBSF is a broad spectrum, irreversible inhibitor of serine proteases [1].AEBSF is a covalently binding inhibitor of proteases, including trypsin, chymotrypsin, plasmin and thrombin. AEBSF was found to inhibit A production in various cell lines. In K293 cells transfected with APP695 (K695sw), AEBSF showed does-dependent reduction of A with IC50 value of about 1mM. In HS695 and SKN695 cells transfected with wild-type APP695, AEBSF showed inhibition effect with IC50 value of about 300 M. AEBSF was also found to increase -cleavege and inhibit – cleavage. Besides that, as a protease inhibitor, AEBSF was reported to prevent monocyte-derived macrophages from lysing the leukemic cells. Incubation of macrophages with 150 M AEBSF for 6 hours resulted in nearly maximum inhibition [1, 2]. References:[1] Citron M, Diehl T S, Capell A, et al. Inhibition of amyloid -protein production in neural cells by the serine protease inhibitor AEBSF. Neuron, 1996, 17(1): 171-179.[2] Nakabo Y, Pabst M J. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. Journal of leukocyte biology, 1996, 60(3): 328-336.

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