Human RXR Reporter Assays PANEL, 3 x 32 assays in 96-well format

Product Overview :
Indigo’s panel of RXR reporter assays uses non-human mammalian cells modified to individually express human retinoic acid receptors, RXRα (NR2B1), RXRβ (NR2B2), or RXRγ (NR2B3). Indigo’s RXR reporter cells include the luciferase reporter gene functionally linked to a responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in RXRα, RXRβ or RXRγ activity. The principal application of this assay panel is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against the three human RXR’s. RXR reporter cells are prepared using Indigo’s specific CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. Indigo Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to RXR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. IB0083

Size : 1 Kit        

Shipping : dry ice  

Stability : Store at -80 degrees; shelf life 180 days maximum after production  

Application(s) : human 

Specificity :

• Retinoid X Receptors (RXRα, RXRβ, RXRγ)

Custom Code : 3822000002 

UNSPSC code : 41116104

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