R-1479

Short Summary : HCV replication inhibitor

Category : Microbiology & Virology|HCV

Purity : 0.98

CAS Number : 478182-28-4

Formula : C9H12N6O5

Molecular Weight : 284.23

SMILE : C1=CN(C(=O)N=C1N)C2C(C(C(O2)(CO)N=[N+]=[N-])O)O

Solubility : Soluble in DMSO

Storage : Store at -20°C

Description : R1479 is a specific inhibitor of HCV replication in cell culture with IC50 value of 1.28 M [1].
Hepatitis C virus (HCV) is an enveloped (+)-single stranded RNA virus, and the viral RNA is replicated in host cell via RNA-dependent RNA polymerase of HIV, which is the cause of hepatitis C and some cancer lymphomas [2].
R1479 is a potent and specific inhibitor of HCV NS5B RNA polymerase to inhibit HCV replication in the HCV subgenomic replicon system. In proliferating replicon cells of 2209-23 cell line, 72 h incubation of R1479 showed inhibition of HCV RNA replication (IC 50 = 1.28 M), which had the similar potency with 2_-C-methylcytidine (IC 50 = 1.13 M). R1479 had no influence on cell proliferation and viability of Huh-7 cells at concentration up to 2 mM, which suggested the inhibition by R1479 was a direct antiviral mechanism [2]. Furthermore, prolong incubation of R1479 for HCV replicon cells led to a continuous reduction of HCV RNA to an undetectable level within 15 days of incubation [2].Finally, R1479 showed no cytotoxicity in replicon cells [1].
References:[1] Smith D B, et al. Design, synthesis, and antiviral properties of 4′-substituted ribonucleosides as inhibitors of hepatitis C virus replication: the discovery of R1479. Bioorg Med Chem Lett., 2007, 17(9): 2570-6.[2] Klumpp K, et al. The novel nucleoside analog R1479 (4′-azidocytidine) is a potent inhibitor of NS5B-dependent RNA synthesis and hepatitis C virus replication in cell culture. J Biol Chem., 2006, 281(7): 3793-9.

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