SAG

Short Summary : Hh and Smo agonist

Category : Stem Cell|Hedgehog

Purity : 0.9825

CAS Number : 912545-86-9

Formula : C28H28ClN3OS

Molecular Weight :

Molecular Weight : 490.06

SMILE : CN[C@@]1([H])CC[C@@](N(C(C2=C(Cl)C3=CC=CC=C3S2)=O)CC4=CC(C5=CC=NC=C5)=CC=C4)([H])CC1

Solubility : >24.5mg/mL in DMSO

Storage : Store at -20°C

Description : SAG is a synthetic chlorobenzothiophene-containing Hh pathway agonist that regulates smo activity. It is a SMO agonist at EC50 of 3 nM and but inhibits hedgehog signaling at >1 M. [1]
Smoothened (SMO) is a GPCR-like receptor that mediates hedgehog signaling with Patched to regulate gene expression through the Gli transcription factors. It is plays an important role for embryogenesis and adults tissue homeostasis
In a mouse cultured cell assay (Shh-LIGHT2), SAG induces pathway activation (EC50 3 nM). It differs in that pathway activity decreases dramatically as SAG concentration surpasses 1 M. [1] SAG binds directly to the SMO heptahelical bundle. SAG dose dependently blocks this association and produces apparent dissociation constant (KD) of 59 nM for the SAG/SMO complex. [2]
SAG also antagonizes GCs antiproliferative effects in CGNP P4 wild-type mice primary cultures. At 15-240 nM, SAG prevents the neurotoxic effects of glucocorticoids without interfering with the beneficial effects of glucocorticoids on lung maturation. [2]
References:1. Chen JK, Taipale J, Young KE et al. Small molecule modulation of Smoothened activity. Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14071-6.2. Heine VM, Griveau A, Chapin C et al. A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Sci Transl Med. 2011 Oct 19;3(105):105ra104.

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