TEMPase HOT START DNA Polymerase Glycerol Free 5 U/µl 1000U (2 tubes x 0.1 mL), 10x Ammonium Buffer (2 tubes x 1.5 mL), 10x Combination Buffer (2 tubes x 1.5 mL) (both Mg2+ free) and 25 mM MgCl2 (2 tubes x 1.5 mL)

TEMPase Hot Start DNA Polymerase Glycerol Free is a glycerol free formulation of our regular TEMPase Hot Start DNA Polymerase and is well suited for automated high throughput testing, freeze drying or where accurate pipetting of small volumes are crucial. TEMPase Hot Start DNA Polymerase Glycerol Free is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase, resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.

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