Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit

Short Summary : Detects alpha-ketoglutarate dehydrogenase activity.

Category : Metabolism Kit|Energy Metabolism Kit

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Storage : Store at -20°C

Description : ?-Ketoglutarate Dehydrogenase (?-KGDH) is an important enzyme in the citric acid cycle. Combining with dihydrolipoamide succinyl transferase (E2) and dihydrolipoamide dehydrogenase (E3), ?-KGDH forms an enzyme complex. In the presence of NAD+ and CoA, ?-KGDH converts ?-ketoglutarate into succinyl-CoA. ?-KGDH is highly regulated by intracellular calcium, NADH/NAD+ and ATP/ADP ratios. Decreased ?-KGDH activity can lead to Alzheimer’s disease. ?-KGDH is also a target of oxidative stress. Reactive oxygen species (ROS) can inhibit ?-KGDH activity which then diminishes its important function and causes a bioenergetic deficit.
The Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a sensitive, simple, fast and convenient way for detection ?-KGDH activity in various samples based on colorimetric method. In the assay, ?-KGDH changes ?-ketoglutarate to an intermediate which reduces the probe to a colored product with strong absorbance at 450 nm. The kit can detect ?-KGDH activity lower than 0.1 mU in a variety of samples.

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