ChromaLink™ One-Shot Antibody Biotinylation Kit – Brilliant!

ChromaLink One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple A280 and A354 measurement determines the number of biotins per antibody molecule.

Highlights:

• Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody
• Fast and easy method to biotinylate and determine the amount of labeling
• Label and determine the extent of biotin labeling with one reagent
• Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
• Nondestructive assay does not destroy biotinylated sample

Applications include:

• Biotinylate antibodies
• Quantitate the extent of biotin labeling
• Optimize biotin-avidin complex.

Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin from SoluLinK accurately determines the degree of biotin incorporation without any of these disadvantages.This reagent, also available as a reagent only in both 5 × 1mg and 10mg formats (B-1007-105 and B-1007-110), contains three important features that make it ideal for biotinylation:

1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.

 

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