Cytoplasmic & Nuclear RNA Purification Kit

Norgen’s Cytoplasmic & Nuclear RNA Purification Kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. I n certain circumstances it is desirable to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling, or nuclear RNA in order to study pre-processed RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. The cytoplasmic RNA is preferentially purified from the nuclear RNA and the other cellular components such as genomic DNA and proteins, without the use of phenol or chloroform. The purified RNA is of the highest quality and can be used in a number of downstream applications.

Features:

Rapid spin-column format allows for the processing of 10 samples in 45 minutes.

Purified cytoplasmic RNA can be used directly in RT-PCR reactions with no DNAse treatment required (Fig 2.)

Cytoplasmic and nuclear RNA is isolated without the use of harmful chemicals such as phenol or chloroform.

All nuclear and cytoplasmic RNA species can be isolated, from large mRNA species down to microRNA (miRNA) and small interfering RNA (siRNA).

Purified RNA can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNAse protection and primer extension, and expression array analysis requiring the use of intact RNA.

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