Double-Strand Specific DNase (dsDNase)
• Double-strand DNA specific endonuclease
• Easily inactivated by heat
• No degradation of primers.
dsDNase is an endonuclease that cleaves phosphodiester linkages in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. dsDNase has a very high specific activity, estimated 30 times higher than bovine DNase I, and it is heat labile. dsDNase has a particularly strong preference for double-stranded DNA (dsDNA). In the presence of magnesium as only divalent cation and using oligos as a substrate; the activity towards dsDNA is 5000-fold higher than towards ssDNA. The enzyme can therefore be used to specifically degrade dsDNA, leaving ssDNA essentially intact.
Source : Recombinantly produced in Pichia pastoris.
Activity : dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Heat inactivation: dsDNase is completely inactivated by incubating at 65°C for 15 min. 1 mM DTT is required for irreversible inactivation.
Storage : Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.
Purity : dsDNase is purified to apparent homogeneity.
Specific activity : 470 000 Kunitz Unit/mg.
Unit definition : One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).
dsDNase-2-250 concentration 2Units/ul 125ul = 250units
dsDNase-2-1000 concentration 2Units/ul 500ul = 1000units
dsDNase-5-2500 concentration 5Units/ul 500ul = 2500units
All prices shown are exclusive of VAT