Double-Strand Specific DNase (dsDNase)


• Double-strand DNA specific endonuclease

• Easily inactivated by heat

• No degradation of primers.

Properties :
dsDNase is an endonuclease that cleaves phosphodiester linkages in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. dsDNase has a very high specific activity, estimated 30 times higher than bovine DNase I, and it is heat labile. dsDNase has a particularly strong preference for double-stranded DNA (dsDNA). In the presence of magnesium as only divalent cation and using oligos as a substrate; the activity towards dsDNA is 5000-fold higher than towards ssDNA. The enzyme can therefore be used to specifically degrade dsDNA, leaving ssDNA essentially intact.
Source : Recombinantly produced in Pichia pastoris.
Activity : dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Heat inactivation: dsDNase is completely inactivated by incubating at 65°C for 15 min. 1 mM DTT is required for irreversible inactivation.
Storage : Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.
Purity : dsDNase is purified to apparent homogeneity.

Specific activity : 470 000 Kunitz Unit/mg.
Unit definition : One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950).

Note :

dsDNase-2-250 concentration 2Units/ul  125ul = 250units

dsDNase-2-1000 concentration 2Units/ul  500ul = 1000units

dsDNase-5-2500 concentration 5Units/ul  500ul = 2500units

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