FFPE RNA/DNA Purification Kit

Norgen’s FFPE RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Alternatively, the kit can be used to isolate total RNA alone or genomic DNA alone from FFPE tissue samples through varying the protease digestion time and performing optional RNase or DNAse digestions . Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE RNA/DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.

FFPE RNA/DNA Kit Benefits :

• High quality and integrity of the isolated nucleic acid – Both the purified total RNA and/or genomic DNA are of the highest quality and integrity, and can be used in any sensitive downstream application (Figures 1 and 2).
• Isolate a diversity of RNA species – All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
• High yields – Norgen’s FFPE RNA/DNA Purification Kit allows for the purification of high yields of total RNA or DNA (Figure 1).
• Rapid procedure – Isolate total RNA and/or genomic DNA from FFPE tissue sections using a rapid spin column format.
• Versatile procedure – The kit allows for the purification of either pure total RNA , pure genomic DNA or both RNA and genomic DNA through the use of tailored protease digestion times.

FFPE RNA/DNA Kit Applications :

• qPCR
• qRT-PCR
• primer extension
• expression array assays
• PCR reactions
• sequencing
• Southern blotting
• SNP analysis

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