FFPE RNA Purification Kit

Norgen’s FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time .

FFPE RNA Kit Benefits :

• High quality and integrity of the isolated RNA – The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications (Figure 1).
• Isolate a diversity of RNA species – All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
• High yields – Norgen’s FFPE RNA Purification Kit allows for the purification of high yields of total RNA (Figure 2).
• No phenol:chloroform extractions – Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
• Rapid procedure – Isolate total RNA from FFPE tissue sections using a rapid spin column format.

FFPE RNA Kit Applications :

• qPCR
• qRT-PCR
• primer extension
• expression array assays

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