HSF1 Rat Monoclonal Antibody (Clone 10H8)

Product : Rat HSF1 antibody; monoclonal

Clone : 10H8

Immunogen : Purified recombinant HSF1 protein

Host and Subclass : Rat immunoglobulin, IgG1

Applications : WB(1), IP, ICC(1), ELISA(1), Gel Mobility Shift Assay (1)

Specificity : Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Species crossreactivity : Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit.

Format : Rat Immunoglobin in PBS, pH 7.4, 0.09% sodium azide in 50% glycerol.

Concentration and working dilution : 1mg/ml; 1µg/ml was sufficient for detection of HSF1 by ECL immunoblot in 20µg of HeLa lysate.

Storage and stability : -20°C; 1 year+; shipped on cold packs or ambient.

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