Human NF-kB Reporter Assay System, 1 x 384-well format assay
Product Overview :
This assay system utilizes HEK293t cells that express NF-kB (nuclear factor kappa-light-chain enhancer of activated B Cells), and contain the luciferase reporter gene functionally linked to upstream NF-kB genetic response elements. Thus, quantifying changes in luciferase expression provides a sensitive surrogate measure of changes in the level of NF-kB activation.
NF-kB is a signal transduction dependent transcription factor. This NF-kB reporter cell line is validated to provide a robust dose-dependent activation response when treated with TNFa, or the Protein Kinase C activator Phorbol 12-myristate 13-acetate (PMA). As such, the principle application of this assay system is in the screening of test samples to quantify any functional activities that they may exert to modulate, either induce or suppress, NF-kB activities.
Size : 1 Kit
Shipping : Dry Ice
Stability : Store at -80 degrees; shelf life 180 days maximum after production
Application(s) : Human
Specificity : Nuclear Factor kappa-light-chain enhancer of activated B cells (NF-kB)
Custom Code : 3822000002
UNSPSC code : 41116104
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