microRNA Purification Kit
Norgen’s microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, and bacterial cells. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
Rapid spin-column format allows for the processing of 10 samples in 30 minutes.
RNA is isolated without the use of harmful chemicals such as phenol or chloroform.
All small RNA species can be isolated including miRNA, siRNA, tRNA and 5S rRNA.
Efficient isolation of small RNA species using a 2 column process, resulting in minimal contamination of larger RNA and genomic DNA.
Purified RNA can be used in a number of downstream applications relating to gene regulation and functional analysis, including northern blotting and microarray analysis.
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