Proteospin CBED (Concentration, Buffer Exchange and Desalting) Micro Kit

Norgen Biotek Corporation

The ProteoSpin™ CBED Micro Kit provides a fast and simple procedure for concentration, buffer exchange and desalting of protein samples. The kit is based on spin-column chromatography using Norgen’s patented protein resin as an ion exchanger. Salts such as MgCl2, NaCl, KCl, CaCl2,LiCl, CsCl have poor affinities for Norgen’s resin and are easily removed from the sample. The elution is performed with choices of several different buffer solutions.

Each spin column can process up to 50 µg of protein from up to 5 mL of solution (Table 1). The kit contains complete solutions for the preparation of both acidic and basic proteins. The ProteoSpin™ CBED Micro Kit provides a convenient method for preparing proteins before running downstream proteomic applications including mass spectrometry, SDS-PAGE, isoelectric focusing, x-ray crystallography and NMR spectroscopy.

ProteoSpin™ CBED (Concentration, Buffer Exchange and Desalting) Kit Benefits :

  • Fast and easy processing – Efficiently process up to 12 samples in only 20 minutes using an easy-to-use protocol.
  • No mixing or formulation – Solutions and protocols are provided for both acidic and basic proteins with no need for formulation.
  • No molecular weight cutoff – Based on an ion exchange mechanism, a broad size range of proteins, from peptides to antibodies can be processed.
  • Proteins bind while salts are discarded in flowthrough – Norgen’s resin has an inherent low affinity for salt, but high affinity for proteins, providing salt removal and buffer exchange simultaneously.
  • Suitable for downstream applications – Final elution performed with volatile or non-volatile buffers suitable for:

• Mass Spectrometry
• SDS-PAGE
• Isoelectric Focusing
• X-ray Crystallography
• NMR Spectroscopy

Proteospin CBED (Concentration, Buffer Exchange and Desalting) Micro Kit
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£185.00 / €259.00 10100

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£289.00 / €404.60 10400

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