Qualitative ELISA for Bt-Cry1Ab/1Ac
B. thuringiensis forms crystals of proteinaceous insecticidal δ-endotoxins (Cry toxins) which are encoded by cry genes. Many genetically engineered crops have been developed with insect tolerance till date by expressing cry genes from Bacillus thuringiensis. Bt-modified crops appear to be safe for farmers and consumers.
The Krishilisa Bt-Cry1Ab/1Ac ELISA is used as an analytical tool for qualitative laboratory screening of presence or absence of Bt-Cry1Ab/1Ac proteins in leaves, seedlings and seeds of transgenic crop varieties like cotton. The assay can be used to detect the presence of these proteins in cotton products including Bollgard™ and Widestrike™.
The Krishilisa Bt-Cry1Ab/1Ac Qualitative kit is an Enzyme-Linked Immunosorbent Assay (ELISA). Plant leaf or seed sample extracts are added to microtiter plate, which is coated with antibodies to Btcy1Ab/1Ac toxin. If Bt-Cry1Ac protein is present in the sample, it binds to the antibody and thus is captured in the wells, which can then be detected by addition of an Enzyme Conjugate. This immunological reaction results in the formation of a sandwich complex of solid phase Antibody-Toxin-Enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure. The Substrate is then added. If any enzyme is present, the Substrate will be hydrolysed signifying the presence of Bt-Cry1Ac protein. This color reaction can be measured spectrophotometrically or observed visually. The color development is proportional to the concentration of Cry1Ac toxin in the sample.
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