RNA/DNA/Protein Purification Plus Micro Kit
For sequential isolation of total RNA, genomic DNA and total proteins from the same sample
- Sequentially purify RNA (and miRNA), DNA and proteins from a single sample
- Small elution volume down to 20 µL with a specialized column
- No sample splitting, no need to use phenol or precipitation methods
- Proteins are purified on column and are soluble in the elution buffer
- Proteins require no further cleaning – ready for Western blot and Mass spectrometry
- Suitable for cells, tissues, stem cells, CTC, small input of samples
- Rapid and efficient spin column procedure – all done in 30 minutes
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC.
The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
The proteins are eluted in buffer, so that there are no pellets to resuspend and the proteins are directly ready for quantification, Western Blots, and Mass spectrometry.
This kit is ideal for researchers who are interested in system biology and are studying the genome, proteome and transcriptome of a single sample. Norgen’s RNA/DNA/Protein Purification Plus Micro Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials, LCM or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results.
The purified RNA, DNA and proteins are of the highest integrity and can be used in a number of downstream applications.
All prices shown are exclusive of VAT